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For example, antibodies precipitate at 40-50% ammonium sulphate. The purity of antibodies precipitated using this method depends on the temperature, pH, the rate at which salt is added, and time.
The proteins were released from the membrane by sonication and purified >900-fold by ammonium sulfate precipitation, gel filtration, and ion-exchange chromatography. The enzymes appear to be the ...
Starting with soluble proteins extracted from the cotyledons of 5-d-old soybean seedlings and a 45% ammonium sulfate precipitation, MS and gMDH coeluted on Superdex 200 HR (low-ionic-strength ...